The importance of viral infections as a leading cause of morbidity and mortality is well documented in severely immunosuppressed patients undergoing allogeneic stem cell transplantation. By contrast, viral infections generally receive less attention in patients with malignant disorders undergoing chemotherapy, where the onset of neutropenic fever is mostly associated with bacterial or fungal infections, and screening for viral infections is not routinely performed. To address the occurrence of invasive viral infections in a clinical setting commonly associated with less pronounced immunosuppression, we have prospectively screened 237 febrile neutropenic episodes in pediatric (n=77) and adult (n=69) patients undergoing intensive chemotherapy, primarily for treatment of acute leukemia. Serial peripheral blood specimens were tested by RQ-PCR assays for the presence and quantity of clinically relevant viruses CMV, EBV, HHV6 and HAdV, commonly reactivated in highly immunocompromised patients. Viremia was documented in 36 (15 %) episodes investigated, including the detection of HHV6 (n=14), EBV (n=15), CMV (n=6), or HAdV (n=1). While low or intermediate levels of viremia (<104 virus copies/ml) were commonly associated with bacterial or fungal co-infection, viremia at higher levels (>104 copies/ml) was documented in patients without evidence for other infections, raising the possibility that at least in some instances the onset of fever may have been attributable to the virus detected. The observations suggest that viral infections, potentially resulting from reactivation, might also play a clinically relevant role in patients receiving chemotherapy for treatment of malignant neoplasms, and routine screening for viremia in this clinical setting might be warranted. This article is protected by copyright. All rights reserved.
Klára Obrová, Silke Grumaz, Marlene Remely, Stefan Czurda, Isabella Krickl, Susanne Herndlhofer, Karoline V Gleixner, Wolfgang R Sperr, Lisa Größlinger, Tijana Frank, Nuno Andrade, Teresa Egger-Matiqi, Christina Peters, Gernot Engstler, Michael Dworzak, Andishe Attarbaschi, Martine van Grotel, Marry M van den Heuvel-Eibrink, Ivan S Moiseev, Yuliya Rogacheva, Ludmilla Zubarovskaya, Natalia Zubarovskaya, Herbert Pichler, Anita Lawitschka, Elisabeth Koller, Felix Keil, Peter Valent, Kai Sohn, Thomas Lion