A Flow cytometric assay to detect platelet activating antibodies in VITT after ChAdOx1 nCov-19 vaccination.

Vaccination is crucial in combatting the SARS-CoV-2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4. We present a widely applicable whole blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories.

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Authors: Stefan Handtke, Martina Wolff, Carlo Zaninetti, Jan Wesche, Linda Schönborn, Konstanze Aurich, Lena Ulm, Nils-Olaf Hübner, Karsten Becker, Thomas Thiele, Andreas Greinacher