It has recently been hypothesized that ketone bodies may have independent cardioprotective effects due to increased myocardial efficiency and that this may explain the improved survival of individuals with type 2 diabetes treated with mildly ketogenic sodium-glucose cotransporter 2 (SGLT2) inhibitors.Are ketone bodies selectively utilized in tissues critical for preservation of conscience and circulation? We investigated the effect of acute hyperketonemia on substrate metabolism in less prioritized tissues such as abdominal organs, adipose tissue and skeletal muscle.Acute, randomized, single-blinded, cross-over design.Ambulatory care.Eight healthy subjects completed the study. Two additional subjects withdrew because of claustrophobia during the scans.Infusions of: 1) saline and 2) ketone bodies during a hyperinsulinemic-euglycemic clamp.Organ-specific glucose and palmitate uptake was determined by dynamic PET/CT scans with 18F-FDG and 11C-palmitate. Blood flow to abdominal organs was measured with 15O-H2O perfusion PET. The study was a performed as a post hoc analysis.We found that ketone body infusion did not affect glucose uptake, palmitate uptake or blood flow to abdominal organs and skeletal muscles.Acute hyperketonemia does not affect glucose or palmitate uptake in skeletal muscle or abdominal tissues, supporting the notion that ketone bodies are selectively used by critical organs such as the heart and brain.